Alekseeva O. М., Krivandin A. V., Shatalova O. V., Krementsova A. V., Kim Y. A. THE INFLUENCE OF MELAFEN ON STRUCTURAL PROPERTIES OF PHOSPHOLIPID MEMBRANES

DOI: http://dx.doi.org/10.15688/jvolsu10.2014.5.2

Alekseeva Olga Mikhaylovna

Candidate of Biological Sciences, Senior Researcher, Institute of Biochemical Physics named after N.M. Emanuel of RAS

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Kosygina St., 4, 119334 Moscow, Russian Federation

Krivandin Aleksey Vladimirovich

Candidate of Physical and Mathematical Sciences, Leading Researcher, Institute of Biochemical Physics named after N.M. Emanuel of RAS

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Kosygina St., 4, 119334 Moscow, Russian Federation

Shatalova Olga Vladimirovna

Researcher, Institute of Biochemical Physics named after N.M. Emanuel of RAS

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Kosygina St., 4, 119334 Moscow, Russian Federation

Krementsova Anna Vladimirovna

Candidate of Physical and Mathematical Sciences, Leading Researcher, Institute of Biochemical Physics named after N.M. Emanuel of RAS

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Kosygina St., 4, 119334 Moscow, Russian Federation

Kim Yuriy Aleksandrovich

Doctor of Physical and Mathematical Sciences, Leading Researcher, Institute for Biological Instrumentation of RAS

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Institutskaya St.,7, 142290 Pushchino, Russian Federation

Abstract. The paper studies the effect of aqueous solutions of melamine salt of phosphonic acid (Melafen), used in crop production, on structural and thermodynamic properties of phospholipid membranes. The multilamellar liposomes formed from individual synthetic dimyristoylphosphatidylcholine (DMPC), or natural phospholipid mixture (egg lecithin) was studied by differential scanning microcalorimetry (DSC) and small-angle X-ray scattering (SAXS).

The method of DSC was used to appraise the influence of Melafen aqueous solutions (10–17 M to 10–3 M) on thermodynamic parameters of lipid melting (temperature, enthalpy and cooperation of phase transition) in suspension of liposomes formed from DMPC. On the basis of DSC data it was concluded that the microdomain structural organization of DMPC membranes was altered by Melafen aqueous solutions as polymodal type.

We did not reveal by the SAXS method any noticeable integral structural changes in membranes of egg lecithin in multilamellar liposomes as well as in their stacking parameters (period and degree of order) in Melafen aqueous solutions (10–21 M to 10–6 M). This range of Melafen concentrations is wider than that used in crop production. Relying on the results of this work it can be inferred that microdomain structure of membranes composed of individual neutral phospholipids is changed in Melafen aqueous solutions at the wide concentration range of Melafen. At the same time, Melafen does not affect the overall structure of lipid membranes composed of a natural phospholipid mixture.

Key words: melafen, liposomes, differential microcalorimetry, small-angle X-ray scattering.

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THE INFLUENCE OF MELAFEN ON STRUCTURAL PROPERTIES OF PHOSPHOLIPID MEMBRANES by Alekseeva O. М., Krivandin A. V., Shatalova O. V., Krementsova A. V., Kim Y. A. is licensed under a Creative Commons Attribution 4.0 International License.

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